Identification and characterization of miRNAs and target genes in developing flax seeds by multigroup analysis

Flax (Linum usitatissimum L.) is an important industrial crop, and its seeds are rich in a variety of functional nutrition health components. Therefore, flaxseed not only raw material agricultural production but also indispensable part to increase the added value flax products. To investigate role miRNA development flaxseed, present study used Shuangya 4 at 10, 20 30 days post anthesis (DPA) as material. The identified 130 known miRNAs 93 new miRNAs; total 3,361 target genes were predicted, including 2401 corresponding 960 miRNAs. Combining sequencing results degradation group, it was found that miR156, miR171, miR164, miR408 miR398 well unconservative_scaffold3872_46150 showed significant differential expression flaxseed. These differentially expressed their analyzed by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). could be negatively regulated contribute flaxseed.Supplemental data for this article available online https://doi.org/10.1080/13102818.2021.1903337 .